Hi Jennifer!
Thank you so much for your question!
You're in LUCK! Your children are just about the same ages as mine, so I can speak from experience!
Magic Kingdom is a big one in our house. Plan on Fantasyland (and New Fantasyland) being a big hit! Also, make sure you see Enchanted Tales with Belle. It's a BIG hit with the girls (and boys as well!). My boys also really enjoy Haunted Mansion as well as Buzz Lightyear's Space Ranger Spin. As far as thrill rides go, they both enjoyed Big Thunder Mountain Railroad, and my oldest loved his first ride on Space Mountain.
At Epcot, they LOVE The Seas with Nemo and Friends as well as Test Track. They also love exploring Innoventions and doing some of the interactive experiences there as well! Spaceship Earth is also a favorite.
At Hollywood Studios, they can't miss Toy Story Midway Mania! We make sure to fit it in several times during our trip. If your two year old is a HUGE Disney Junior fan (like ours), then DON'T MISS Disney Junior - Live on Stage! She'll be boppin' and singing and dancing along! Our boys have both experienced Tower of Terror, and they aren't big thrill seekers, so they've decided to sit that one out for a couple more years!
Here's some of our favorites at Disney's Animal Kingdom as well!
Have a magical vacation!
Amanda